Medicago truncatula Handbook

ISBN 0-9754303-1-9
Preface to the Medicago truncatula Handbook

Biology of Medicago truncatula

• Morphology, development and plant architecture of M. truncatula
  Vegetative development (D. Moreau)
• Evolutionary and Ecological Genetics of Medicago truncatula (T. Bataillon, J. Ronfort)
• Seed Biology of Medicago truncatula (K. Gallardo, C. Lesignor, M. Darmency, J. Burstin, R. Thompson, C. Rochat, J.-P. Boutin, H. Kuester, J. Buitink, O. Leprince, A. Limami, M.A. Grusak)
• Response of Medicago truncatula to abiotic stress (A.M. Limami, C. Ricoult, E. Planchet, E.M. González, R. Ladrera, E. Larrainzar, C.Arrese-Igor, F. Merchan, M. Crespi, F. Frugier, K. Avia, I. Lejeune-Hénaut)
• Symbiotic interactions
  • Arbuscular mycorrhiza (V. Gianinazzi-Pearson, I. Maldonado-Mendoza, M. Lopez-Meyer, S. Weidmann and M. J. Harrison)
• Pathogenic interactions
  • Fungi (S. Ellwood, J. Lichtenzveig, T. Pfaff, L. Kamphuis, R. Oliver)
  • Medicago truncatula as a model for studying interactions between root parasitic plants and legumes (A. Pérez-de-Luque, M.D. Lozano, A.M. Maldonado, J.V. Jorrín, M.A. Dita, J. Die, B. Román, D. Rubiales)

M. truncatula resources

• M. truncatula stock centre (R. Nair , S.J. Hughes, S. Ellwood, R. Oliver, S.L. Green, M. Delalande, J. Wen, G. Oldroyd)
• Wild accessions/populations (S. Greene, S.J. Hughes, R. Nair, T. Huguet, M.E. Aouani, J.M. Prosperi, M. Delalande)
• Medicago truncatula cultivars (R. Nair, J. Howie, M. Delalande)
• Bionformatics resources for Medicago truncatula - 2006
  • UrMeLDB: The European Medicago genome database (K. Mayer et al.)
  • Medicagoo: Searching Medicago resources (J. Gouzy et al.)
  • EST analysis and expression profiling software from Bielefeld University (H. Küster et al.)
  • The Legume Information System (LIS): An integrated, dynamic comparative legume information resource (G.D. May et al.)
  • MtDB and medicago.org (E. Retzel, et al.)
  • The Medicago truncatula Genome Sequencing Website (N.D. Young, et al.)
• Medicago truncatula cDNA and genomic libraries (P. Gamas, F. Debellé, H. Berges, L. Godiard, A. Niebel, E.P. Journet, J. Gouzy)
• Mutant gene nomenclature in Medicago truncatula J. Frugoli)

M. truncatula protocol chapters

Handling Medicago truncatula as a whole plant: growth, propagation, crossings, as well as abiotic stress and interactions with other organisms

• Overview (E-P Journet)
• Seed storage and germination (J. Garcia, D.G. Barker and E-P Journet)
• Growing M. truncatula: choice of substrates and growth conditions (D.G. Barker, T. Pfaff, D. Moreau, E. Groves, S. Ruffel, M. Lepetit, S. Whitehand, F. Maillet, R. M. Nair, E-P Journet)
• Vernalization, crossings and testing for pollen viability (M. Chabaud, J. Lichtenzveig, S. Ellwood, T. Pfaff, E-P Journet)
• Medicago truncatula hybridization – supplemental videos (M. Taylor, L. Blaylock, J. Nakashima, D. McAbee, J. Ford, M. Harrison, M. Udvardi)
• Phenotyping (D. Moreau, V. Bourion, S. Fournier, H. De Larambergue, G. Duc, C. Salon, A.S.Voisin, C. Jeudy)
• Cuttings and grafts (S. Cros-Arteil, T. Pfaff, D.G. Barker and E-P Journet)
• Rhizobial inoculation and nodulation of Medicago truncatula (E-P Journet, F. de Carvalho-Niebel, A. Andriankaja, T. Huguet, D.G. Barker)
• Inoculation and growth with mycorrhizal fungi (M. Chabaud, M. Harrison, F. de Carvalho-Niebel, G. Bécard, D G. Barker)
• Inoculation and growth with soil borne pathogenic fungi (J. Lichtenzveig, J. Anderson, G. Thomas, R. Oliver, K. Singh)
• Inoculation and growth with foliar pathogenic fungi (S. Ellwood, L. Kamphuis, T. Pfaff, R. Oliver, D. Samac, D. Foster-Hartnett, B. Tivoli, C. Onfroy, A. Moussart, Villegas A.M., Sillero J.C., Rubiales D.)
• Inoculation and growth with root parasitic weeds (M. Fernández-Aparicio, A. Pérez-de-Luque, M.D. Lozano, D. Rubiales)
• Medicago truncatula resistance to Oomycetes (A. Moussart, B. Tivoli, D. Samac, N. D'Souza)
• Medicago truncatula interaction with insects (O. Edwards, J. Klingler, L. Gao, K. Korth, K. Singh)

Cytology and microscopy

• Immunolocalization (B. Hause, F. Frugier, M. Crespi)
• Flow cytometry (ploidy determination, cell cycle analysis, DNA content per nucleus) (S.J. Ochatt)

Transformation and tissue culture

• Cell suspension cultures (A. Iantcheva, M. Vlahova, A. Atanassov, A. S. Duque, S. Araújo, D. F. dos Santos, P. Fevereiro)
• Agrobacterium rhizogenes-mediated root transformation (M. Chabaud, A. Boisson-Dernier, J. Zhang, C.G. Taylor, O. Yu, D.G. Barker)
• Agrobacterium tumefaciens-mediated transformation and in vitro plant regeneration of M. truncatula (M. Chabaud, P. Ratet, S. de Sousa Araújo, A.S. Roldão Lopes Amaral Duque, M. Harrison, D.G. Barker)
• Somatic embryogenesis from leaf explants (A. Iantcheva, M. Vlahova, A. Atanassov)

Genetic mapping

• Comparative mapping (A. Seres, G. Deák, G. Tóth, G. Aubert, J. Burstin, N. Ellis, G. B. Kiss)

Tools for gene identification, silencing and knockout and mutant screening

• Mutant generation using gamma rays (G. Duc, A. Pelletier)
• Insertional mutagenesis in M. truncatula using Tnt1 retrotransposon (P. Ratet, A. Porcedu, M. Tadege, K.S. Mysore)
• Mutant screening/phenology key (D. Moreau and N. Munier-Jolain)
• Sharing phenotypic data: A coding system and a developmental model (J. Hanan, B. Bucciarelli, C.P. Vance)

Functional genomics

• Transcriptomics (H. Küster, A. Becker, D. Samac, M. Tesfaye)
• Proteomics (S. Nagaraj, Z. Lei, B. Watson, L. Sumner, K. Gallardo, E. Dumas-Gaudot, G. Recorbet, F. Robert, O. Thiery, B. Valot, U. Mathesius, E. Triplett)