Associate Professor Kirankumar S. Mysore
Kirankumar S. Mysore, Ph.D
Associate Professor
Noble Researcher since 2002
curriculum vitae · biosketch
Ph.D., Genetics, 1999, Purdue University
Research emphasis: Molecular plant-microbe interactions and plant genomics
One of the main projects in the Mysore laboratory focuses on understanding the nonhost pathogen resistance mechanism in plants. Non-host resistance has been a topic of interest for many plant pathology researchers for several years. So far, very little research has been done to understand the phenomenon of non-host resistance. They are using Nicotiana benthamiana and Arabidopsis thaliana as model plants to understand nonhost pathogen resistance. Several functional genomic approaches like virus-induced gene silencing (VIGS) and gene expression profiling are being used in these projects. By identifying the signal transduction components of nonhost resistance we can better understand how plants defend against pathogens. In future, this will help us to genetically engineer crop plants for broad resistance to several pathogens.
The Mysore laboratory is also interested in understanding Agrobacterium-plant interactions during Agrobacterium-mediated plant transformation. We seek to identify plant genes that are required for Agrobacterium-mediated plant transformation. We are using N. benthamiana and Arabidopsis as model plants for this project. Identification of plant genes involved in Agrobacterium-mediated plant transformation will help us to increase the transformation efficiency of transformation recalcitrant crops like Soybean and Maize. Understanding Agrobacterium-plant interaction will also help us to control the crown gall disease that is common in grapes and some ornamental plants.
The Mysore laboratory is generating genetic resources of the model legume, Medicago truncatula. In collaboration with Dr. Pascal Ratet, CNRS, France, we are currently doing a large-scale insertional mutagenesis in M. truncatula using a tobacco retrotransposon, Tnt1, which is a viable and attractive option to introduce multiple independent insertions per plant for saturation mutagenesis. They have already generated thousands of transposon tagged M. truncatula lines and we perform a screening event annually to identify desired plant mutants. If you are interested to participate in the screening event please contact Dr. Kiran Mysore.
Uppalapati, S. R., Ishiga, Y., Doraiswamy, V., Bedair, M., Mittal, S. , Chen, J. , Nakashima, J., Tang, Y., Tadege, M., Ratet, P., Chen, R., Schultheiss, H., & Mysore, K. S. (2012). Loss of abaxial leaf epicuticular wax in Medicago truncatula irg1/palm1 mutants results in reduced spore differentiation of anthracnose and nonhost rust pathogens. Plant Cell, January, doi/10.1105/tpc.111.093104.
Tadege, M., Lin, J., Niu, L., & Mysore, K. S. (2011). Control of dicot leaf blade expansion by a WOX gene, STF (Addendum). Plant Signaling & Behavior, 6 (11), 1861-1864.
Senthil-Kumar, M., & Mysore, K. S. (In Press).New dimensions for VIGS in plant functional genomics. Trends in Plant Science. TRPLSC-905, doi:10.1016/j.tplants.2011.08.006.
Ishiga, Y., Ishiga, T., Uppalapati, S. R., & Mysore, K. S. (2011). Arabidopsis seedling flood-inoculation technique: A rapid and reliable assay for studying plant-bacterial interactions. Plant Methods, 7 (32), doi:10.1186/1746-4811-7-32.
Muthappa, S-K., & Mysore, K. S. (2011). Virus-induced gene silencing can persist for more than 2 years and also be transmitted to progeny seedlings in Nicotiana benthamiana and tomato. Plant Biotechnology Journal, 9 (7), 1-10 doi:10.1111/j.1467-7652.2011.00589.x.
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