Gail L. Shadle
Plant Biology Division
Samuel Roberts Noble Foundation
2510 Sam Noble Parkway
Ardmore, OK  73401 USA

Phone: (580) 224-6118
E-mail: glshadle@noble.org

• Research Associate. Samuel Roberts Noble Foundation, Plant Biology Division. 

• Downregulation of key enzymes in the lignin pathway in alfalfa and analysis of the resulting genetically modified plants.
• Developing a high throughput method for transforming Medicago truncatula.

• B.S. Biology/Chemistry, 1999
  Lock Haven University of Pennsylvania, Lock Haven, PA
  GPA: 3.89

• Research Associate. Samuel Roberts Noble Foundation, Plant Biology Division. January 2006 to present.
• Sr. Research Assistant. Samuel Roberts Noble Foundation, Plant Biology Division. July 2005 to December 2005.
• Research Assistant. Samuel Roberts Noble Foundation, Plant Biology Division. June 1999 to July 2005.
• Laboratory Assistant. Lock Haven University of Pennsylvania, Cellular and Molecular Biology laboratory. August through December 1998.
• Laboratory Assistant. Lock Haven University of Pennsylvania, Biochemistry laboratory. January through May 1999.

• Gail L. Shadle, Fang Chen, Lisa A. Jackson, and Richard A. Dixon. “Down-regulation of cinnamyl alcohol dehydrogenase and its impact on forage quality in alfalfa (Medicago sativa).”  American Society of Plant Biologists, July 7-11, 2007, Chicago, IL.
• Lisa A. Jackson, Fang Chen, Jin Nakashima, Gail L. Shadle, and Richard A. Dixon. “Cinnamoyl CoA reductase down-regulation and its impact on forage quality in alfalfa (Medicago sativa).”  American Society of Plant Biologists, July 7-11, 2007, Chicago, IL.
• Gail L. Shadle, Fang Chen, Jin Nakashima, Lisa A. Jackson, M.S. Srinivasa Reddy, and Richard A. Dixon. "EFFECTS OF DOWN-REGULATION OF HCT ON LIGNIN IN ALFALFA." Phytochemical Society of North America Annual Meeting, July 8-12, 2006, Oxford, MS.
• L. Jackson, F. Chen, J. Nakashima, G. Shadle, M.S.S Reddy, and R. Dixon. Studying monolignol biosynthesis in alfalfa by the down-regulation of Cinnamoyl CoA reductase. Phytochemical Society of North America Annual Meeting, July 8-12, 2006, Oxford, MS.
• Jin Nakashima, M.S. Srinivasa Reddy, Fang Chen, Gail Shadle, Lisa Jackson, and Richard A. Dixon. "Down-regulation of key enzymes in the monolignol pathway: effects on lignin composition in specific cell types of alfalfa (Medicago sativa L.)" Phytochemical Society of North America Annual Meeting, July 8-12, 2006, Oxford, MS.
• Gail L. Shadle, M.S. Srinivasa Reddy, Lisa A. Jackson, Fang Chen, and Richard A. Dixon. "Characteristics of alfalfa (M.sativa) down-regulated in expression of ferulate-5-hydroxylase or cinnamate-4-hydroxylase." American Society of Plant Biologists, July 16-20, 2005, Seattle, WA.
• M.S. Srinivasa Reddy, Fang Chen, Gail Shadle, Lisa Jackson, and Richard A. Dixon. "Understanding monolignol biosynthesis in Medicago sativa by a reverse genetics approach." American Society of Plant Biologists, July 24-28, 2004, Orlando, FL.
• Lisa Jackson, Gail Shadle, M.S. Srinivasa Reddy, Fang Chen, and Richard A. Dixon. "Understanding monolignol biosynthesis in Medicago by downregulation of p-coumarate 3-hydroxylase." Society for In Vitro Biology Annual Meeting, May 22-26, 2004, San Francisco, CA.
• Gail Shadle, Lisa Jackson, M.S. Srinivasa Reddy, Fang Chen, and Richard A. Dixon. "Modification of Lignin in Medicago by Down-Regulation of Ferulate 5-Hydroxylase." Society for In Vitro Biology Annual Meeting, May 22-26, 2004, San Francisco, CA.
• He, X.Z., Suzuki, H., Achnine, L., Shadle, G., Jackson, L., Clouse, J. and Dixon, R.A. "Characterization of squalene epoxidase promoter in tobacco cell suspension cultures." XVth International Plant Protection Congress, May 11-16, 2004, Beijing, China.
• Lisa Jackson, Gail Shadle, M.S. Srinivasa Reddy, Fang Chen, and Richard A. Dixon. "A reverse genetics approach to lignin modification in Medicago." Phytochemical Society of North America Annual Meeting, August 9-13, 2003, Peoria, IL.
• X.Z. He, H. Suzuki, L. Achnine, G. Shadle, J. Clouse, and R.A Dixon. "Differential regulation of squalene epoxidase in response to methyl jasmonate induction in tobacco and alfalfa cell suspension cultures." Gordon Conference in Agricultural Science, April 2003, Ventura, CA.
• Gail Shadle, M.S. Srinivasa Reddy, Lisa Jackson, Fang Chen, and Richard A. Dixon. "A reverse genetics approach to study the biosynthesis of monolignols in Medicago." Second Oklahoma Minisymposium in Molecular Plant Biology, March 20-21, 2003, Stillwater, OK.
• B.E. Deavours, C. Steele, G. Shadle, and R.A. Dixon. "Metabolic engineering of isoflavonoid biosynthesis in alfalfa." Second Oklahoma Minisymposium in Molecular Plant Biology, March 20-21, 2003, Stillwater, OK.
• Gail L. Shadle, S. Varsha Wesley, Kenneth L. Korth, Fang Chen, Chris Lamb, and Richard A. Dixon. "Over-expression of L-phenylalanine ammonia-lyase improves plant resistance to viral, fungal, and bacterial pathogens." XXI International Conference on Polyphenols, September 9-12, 2002, Marrakech, Morocco.
• Christy Boydston, Lisa A. Riner, Gail L. Shadle, Stacy N. Allen, Richard A. Dixon, Greg D. May, Nancy L. Paiva, Maria J. Harrison. "Insertional mutagenesis of Medicago truncatula." 4th Workshop on Medicago truncatula, July 7-10, 2001, Madison, WI.

• Jackson, L.A., Shadle, G.L., Zhou, R., Nakashima, J., Chen, F., and Dixon, R.A. (2008). Improving saccharification efficiency of alfalfa stems through modification of the terminal stages of monolignol biosynthesis. BioEnergy Research, in review.
• Nakashima, J., Chen, F., Jackson, L., Shadle, G., and Dixon, R.A. (2008). Multi-site genetic modification of monolignol biosynthesis in alfalfa (Medicago sativa L.) : effects on lignin composition in specific cell types. New Phytologist 179, 738-750.
• Gail Shadle, Fang Chen, M.S.Srinivasa Reddy, Lisa Jackson, Jin Nakashima, Richard A. Dixon. (2007). Down-regulation of hydroxycinnamoyl CoA:Shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage quality. Phytochemistry 68, 1521-1529.
• Fang Chen, M.S Srinivasa Reddy, Stephen Temple, Lisa Jackson, Gail Shadle and Richard A. Dixon (2006) Multi-site genetic modulation of monolignol biosynthesis suggests new routes for the formation of syringyl lignin and wall-bound ferulic acid in alfalfa (Medicago sativa L.). Plant Journal 48, 113-124.
• Reddy, M.S.S., Chen, F., Shadle, G., Jackson, L., Aljoe, H. and Dixon, R.A. (2005). Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.). Proceedings of the National Academy of Sciences USA 102, 16573-16578.
• Gail L. Shadle, S. Varsha Wesley, Kenneth L. Korth, Fang Chen, Chris Lamb, and Richard A. Dixon. (2003). Phenylpropanoid compounds and disease resistance in transgenic tobacco with altered expression of L-phenylalanine ammonia-lyase. Phytochemistry 64, 153-161.
• Hideyuki Suzuki, Yiji Xia, Robin Cameron, Gail Shadle, Jack Blount, Chris Lamb, and Richard A. Dixon. (2003). Signals for local and systemic responses of plants to pathogen attack. Journal of Experimental Botany 55(395), 169-179.