NSF Project: Experimental
| Yeast Elicitation | UV Elicitation | Methyljasmonate Elicitation | Biological Tissue |
Methyljasmonate-Induced Time Course
- A 50mM methyljasmonate stock solution (in ethanol) was used at a 1:100 dilution (2.5ml) for a final concentration of 500uM.
- 2.5ml of ethanol was added to the control flasks.
- The cultures were 9P, or in the 9th passage of subculturing.
- The time course was initiated on Wednesday, April 10, 2002, with the final 48 hour culture harvested on Friday, April 12, 2002.
- The 0 time point was initiated at 9:15AM; the final methyljasmonate treatment was done by 9:40AM (25 minutes from treatment start to treatment finish).
- The yeast elicitor internal controls were initiated at 10:01, 10:02, and 10:03AM.
- The UV internal controls were initiated at 10:25 and 10:30AM.
- All time point harvests were relatively uneventful and as scheduled/planned.
- For a full detailed description of inoculum times and harvest times, see the attached spreadsheet.