NSF Project: Experimental

Yeast Elicitor Time Course

A 5mg/ml yeast elicitor stock solution (in water) was used at a 1:100 dilution (2.5ml) for a final concentration of 50ug glucose equivalents/ml (Yeast elicitor prepared as described in Schumacher et al. 1987).
2.5ml of water was added to the control flasks
The cultures were 11P, or in the 11th passage of subculturing
The time course was initiated on Wednesday, May 8, 2002, with the final 48 hour culture harvested on Friday, May 10, 2002
The 0 time point was initiated at 9:10AM; the final yeast elicitor treatment was done by 9:26AM (16 minutes from treatment start to treatment finish)
The methyljasmonate internal controls were initiated at 9:29, 9:30, and 9:30AM
The UV internal controls were initiated at 11:19 and 11:27AM
All time point harvests were relatively uneventful and as scheduled/planned EXCEPT the 48 hour time point in which the lab lost house vacuum. This problem was overcome by manually applied vacuum; the samples were a bit wetter than the other time points
For a full detailed description of inoculum times and harvest times, see the attached spreadsheet