Kirankumar S. Mysore, Ph.D. Associate Professor Ph.D., Genetics, 1999, Purdue University Joined the Noble Foundation in 2002 Email: ksmysore@noble.org Phone: (580) 224-6740 Fax:     (580) 224-6692

Adjunct faculty appointment: Oklahoma State University, Dept. of Plant Pathology

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Research Emphasis: Molecular plant-microbe interactions and plant genomics

          One of the main projects in my laboratory focuses on understanding the nonhost pathogen resistance mechanism in plants. Nonhost resistance has been a topic of interest for many plant pathology researchers for several years. So far, very little research has been done to understand the phenomenon of non-host resistance. We are using Nicotiana benthamiana and Arabidopsis thaliana as model plants to understand nonhost pathogen resistance. Several functional genomic approaches like virus-induced gene silencing (VIGS) and gene expression profiling are being used in these projects. By identifying the signal transduction components of nonhost resistance we can better understand how plants defend against pathogens. In future, this will help us to genetically engineer crop plants for broad resistance to several pathogens.
          My laboratory is also interested in understanding Agrobacterium-plant interactions during Agrobacterium-mediated plant transformation. We seek to identify plant genes that are required for Agrobacterium-mediated plant transformation. We are using N. benthamiana and Arabidopsis as model plants for this project. Identification of plant genes involved in Agrobacterium-mediated plant transformation will help us to increase the transformation efficiency of transformation recalcitrant crops like Soybean and Maize. Understanding Agrobacterium-plant interaction will also help us to control the crown gall disease that is common in grapes and some ornamental plants.
          My laboratory is generating genetic resources of the model legume, Medicago truncatula. In collaboration with Dr. Pascal Ratet, CNRS, France, we are currently doing a large-scale insertional mutagenesis in M. truncatula using a tobacco retrotransposon, Tnt1, which is a viable and attractive option to introduce multiple independent insertions per plant for saturation mutagenesis. We have already generated thousands of transposon tagged M. truncatula lines and we perform a screening event annually to identify desired plant mutants. If you are interested to participate in the screening event please contact Dr. Kiran Mysore.

Selected Recent Publications:

     Kang, L., Wang, Y-S., Uppalapati S. R., Wang, K., Tang, Y., Vadapalli, V., Venables, B. J., Chapman, K. D., Blancaflor, E. B., and Mysore, K. S. 2008. Overexpression of a fatty acid amide hydrolase compromises innate immunity in Arabidopsis. Plant Journal, in press.

     Tadege, M., Wen, J., He, J., Tu, H., Kwak, Y., Eschstruth, A., Endre, G., Zhao, P. X., Chabaud, M., Ratet, P., and Mysore, K. S. 2008. Large scale insertional mutagenesis using the Tnt1 retrotransposon in the model legume Medicago truncatula. Plant Journal, 54:335-347.

     Uppalapati, S. R., Ishiga, Y., Wangdi, T., Urbanczyk-Wochniak, E., Ishiga, T., Mysore, K. S., Bender, C. L. 2008. Pathogenicity of Pseudomonas syringae pv. tomato on tomato seedlings: Phenotypic and gene expression analyses of the virulence function of coronatine. Molecular Plant-Microbe Interactions, 21:383-395.

     Anand, A., Ryu, C. M., Uppalapati, S. R., Allen, S., N., Kang, L., Tang, Y., and Mysore, K. S. 2008. Salicylic acid and systemic acquired resistance play a role in attenuating crown gall disease caused by Agrobacterium tumefaciens. Plant Physiology, 146:703-715.

     Senthil-Kumar, M., Hema, R., Anand, A., Kang, L., Udayakumar, M., and Mysore, K. S. 2007. A systematic study to determine the extent of gene silencing in Nicotiana benthamiana and other Solanaceae species when heterologous gene sequences are used for virus-induced gene silencing. New Phytologist, 176:882-791.

     Anand, A., Krichevsky, A., Schornack, S., Lahaye, T., Tzfira, T., Tang, Y. Citovsky, V. and Mysore, K. S. 2007. VIP2- a VirE2 interacting protein is required for Agrobacterium T-DNA integration in plants. Plant Cell, 19:1695-1708.

     Uppalapati, S. R., Ishiga, Y., Anand, A., Wangdi, T., Kunkel, B., Mysore, K. S., and Bender, C. L. 2007. The phytotoxin coronatine contributes to pathogen fitness and is required for suppression of salicylic acid accumulation in tomato inoculated with Pseudomonas syringae pv. tomato DC3000. Molecular Plant-Microbe Interactions, 20:955-965.

     Wang, K. Kang, L., Anand, A. and Mysore, K. S. 2007.  Monitoring in planta bacterial infection and population at both cellular and whole plant levels using GFPuv. New Phytologist, 174:212-223. (Cover page article).

     Anand, A., Vaghchhipawala, Z., Ryu, C. M., Kang, L., Wang, K., del-Pozo, O., Martin, G. B., and Mysore, K. S. 2007.  Identification and characterization of genes involved in Agrobacterium-mediated plant transformation by virus-induced gene silencing. Molecular Plant-Microbe Interactions, 20:41-52.

     Senthil-kumar, M., Govind, G., Kang, L., Mysore, K. S., and Udayakumar, M. 2007. Functional characterization of Nicotiana benthamiana homologs of peanut water deficit-induced genes by virus-induced gene silencing. Planta, 225(3):523-39.

     Xu, P., Zhang, Y., Kang, L., Roossinck, M. J. and Mysore, K. S. 2006. Computational estimation and experimental verification of off-target silencing during post-transcriptional gene silencing in plants. Plant Physiology, 142:429-440.

     Tadege, M., Ratet, P. and Mysore, K. S. 2005. Insertional mutagenesis: a Swiss Army Knife for functional genomics of Medicago truncatula. Trends in Plant Science, 10:229-235.

     Ryu, C. M., Anand, A., Kang, L., and Mysore, K. S. 2004. Agrodrench: a novel and effective agroinoculation method for virus-induced gene silencing in roots and diverse Solanaceous species. Plant Journal, 40:322-331.

     Kang, L., Tang, X., and Mysore, K. S. 2004. Pseudomonas type III effector AvrPto suppresses the programmed cell death induced by some nonhost pathogens in Nicotiana benthamiana and tomato. Molecular Plant-Microbe Interactions, 17:1328-1336.

     Anand, A., Lei, Z., Sumner, L. W., Mysore, K. S., Arakane, Y., Bockus, W. W., and Muthukrishnan, S. 2004. Pathogenesis-related proteins from apoplastic fluid of a transgenic wheat line exhibit combinatorial antifungal activity. Molecular Plant-Microbe Interactions, 17:1306-1317.

     Ryu, C. M., Murphy, J. F., Mysore, K. S., and Kloepper, J. W. 2004. Plant growth-promoting rhizobacteria protect Arabidopsis thaliana against Cucumber mosaic virus by a novel signaling pathway. Plant Journal, 39: 381-392.

     Mysore, K. S., and Ryu, C. M. 2004. Nonhost resistance: how much do we know? Trends in Plant Science, 9: 97-104.