Cellular Imaging Facility: Resources

BioRad MRC 1024 ES Confocal Laser Scanning Microscope

Leica TCS SP2 AOBS Confocal Laser Scanning Microscope

Zeiss P.A.L.M. Laser Capture Microdissection Station

Nikon Microphot-FX

Nikon SMZ 1500 - Fluorescent stereo microscope

Olympus SZX 12 - Fluorescent stereo microscope

Nikon Optiphot-2

Ratio and Time Lapse Imaging Station

Vibratome Series 1000

Cryostat Leica CM 1850

Leica RM 2255

BioRad MRC 1024 ES Confocal Laser Scanning Microscope more details

Location: Microscopy Suite

Description: Recently upgraded confocal microscope using the more conventional concepts of mirrors and filters to select for specific wavelengths

Laser: Krypton/Argon

Laser lines: 488, 568 and 647nm

Microscope: Zeiss Axioskop - upright

Objectives:
2.5X Plan-NEOFLUAR NA 0.075 - dry
10X Plan-NEOFLUAR NA 0.3 - dry
25X Plan-NEOFLUAR NA 0.8 - water
40X C-APOCHROMAT NA 1.2 - water
63X C-APOCHROMAT NA 1.2 - water

Software platform: LaserSharp 2000

Software capabilities: triple channel scanning (simultaneous or sequential scanning) plus transmitted light, time series, Z-series, several 3D projection modes (3D reconstruction, max brightness, average, threshold, and etc)

File format: PIC (export capabilities to TIF, JPG, AVI movies)

Compatible fluorophores: GFP, Bodipy, FITC, Alexa 488, YFP, Lucifer Yellow, CY3, TRITC, dsRed, Alexa 568, Texas Red, Propidium Iodide, CY5

Incompatible fluorophores: DAPI, CFP, BFP

Leica TCS SP2 AOBS Confocal Laser Scanning Microscope more details

Location: Microscopy Suite

Description: This system uses state-of-the-art confocal technology such as the acousto-optical beam splitter to select specific wavelengths. Equipped with 4 different lasers, this system is completely tunable and compatible with virtually all fluorophores

Lasers: UV, Argon / Argon Krypton, Helium Neon 543 / 594 and Helium Neon 633

Laser lines: 405, 458, 476, 488, 496, 514, 543, 594 and 633nm

Microscope: Leica DM IRE2 - Inverted, set up for Phase and Nomarski optics

Objectives:
10X HC PL APO NA 0.4 - dry
20X HC PL APO NA 0.7 - dry
63X HCX PL APO NA 1.2 - water
63X HCX PL APO NA 1.4 - oil

Software platform: Leica Confocal Software (LCS)

Software capabilities: quadruple channel scanning (simultaneous or sequential) plus transmitted light, spectral un-mixing of signals, spectral scanning, time series, Z-series, 3D projections and reconstructions, stereo animations and projections, quantification (generates statistical reports, histograms, and etc)

System applications: FRAP, FLIP, FRET, spectral scans

File format: TIF, export capabilities to AVI movie

Fluorophore compatibility: compatible with all fluorophores because the system is completely tunable and programmable

Zeiss P.A.L.M. Laser Capture Microdissection Station more details: Location: Microscopy Suite
Description: This microscope system uses a focused cold laser beam to excise portions of a tissue sample or section. These cut sections which can be as small as one to several cells, are then laser catapulted from the sample into a collection tube for RNA or protein for metabolite analysis.

Nikon Microphot-FX more details

Location: Microscopy Suite - West rooms (North)

Description: This is a wide field compound (upright) microscope set up to do high quality Normarski, Phase Contrast, and Polarization Microscopy. This microscope is also set up to do epifluorescence

Objectives:
4X Plan NA 0.13
10X Plan NA 0.3
20X Plan NA 0.5 DIC
20X Plan NA 0.5
40X MPlan NA 0.5
60X PlanAPO NA 0.95

Filter sets: DAPI, FITC, TRITC

Camera: Nikon DXM 1200 Digital Camera, run by ACT-1 software

Nikon SMZ 1500 - Fluorescent stereo microscope more details

Location: Microscopy Suite - West rooms (South)

Description: This setup is a dissecting microscope, equipped with a fluorescence light source, which allows for quick screening for plants with fluorescent gene expression

Objectives:
0.5X HR PlanApo WD 136
1X HR PlanApo WD 54
1.6X HR PlanApo WD 24

Filter sets: FITC, Rhodamine, CFP

Olympus SZX 12 - Fluorescent stereo microscope more details

Location: Microscopy Suite - West rooms (South)

Description: This setup is a dissecting microscope, equipped with fluorescence, which allows for quick screening for plant with fluorescent gene expression

Objectives:
1X PF DF PLAPO
1.6X PF DF PLAPO

Camera: Nikon DXM 1200C Digital Camera, run by ACT-1 software

Nikon Optiphot-2 more details

Location: Lablink Room 679

Description: Compound, upright microscope for standard wide field applications. This microscope is also set up to do epifluorescence

Objectives:
4X Plan NA 0.13
10X Plan NA 0.3
20X PlanFluor NA 0.5
40X Fluor NA 1.3
60X M Plan NA 0.7

Filter sets: DAPI, FITC, TRITC

Camera: Spot RT color, run by Spot Basic and Spot Advanced software

Ratio and Time Lapse Imaging Station more details

Location: Lablink Room 679

Description: This set up facilitates wide field time lapse imaging of live cells in transmitted light as well as fluorescence. The Princeton Instruments camera with its high dynamic range allows ratiometric and quantitative ion imaging.

Microscope: Nikon Eclipse TE 300 - Inverted

Objectives:
10X PlanFluor NA 0.3 Ph1 DLL - dry
20X PlanFluor NA 0.5 DIC M - dry
40X PlanFluor NA 1.3 DIC H - oil
100X PlanFluor NA 1.3 DIC H - oil

Cameras:
Leica DC 200 (for time lapse)
Princeton Instruments (high dynamic range for quantitative fluorescence and ratiometric imaging)

Software platform: Metamorph and Metafluor

Software capabilities: time lapse, fluorescence quantification, track objects, area-line measurements

System applications: time lapse (transmitted light and epifluorescence), ratiometric ion imaging

File format: STK, INF, export capabilities to TIF and AVI movie files

Vibratome Series 1000 more details: Location: Microscopy Suite - West rooms (North) Temporary location
Microtome for 20-100Î¼m thickness

Vibratome 1000 plus more details: Location: Microscopy Suite - West rooms (North) Temporary location
Microtome for 20-100μm thickness

Cryostat Leica CM 1850 more details: Location: Room 685
Freezing microtome for 10-60Î¼m thickness

Leica RM 2255 more details: Location: Microscopy Suite Temporary location
Microtome for 0.5-100μm thickness

Reichert-Jung Supercut 2050 more details: Location: Lablink Room 679
Ultra microtome for both light and transmission electron microscope

Ultramicrotome RMC more details: Location: Lablink Room 679
Ultra microtome for both light and transmission electron microscope